Recombinant Human NECTIN3 protein, Fc-tagged

• Cat.No.: NECTIN3-0634H
• Product Overview: Human Nectin-3 / CD113 Protein, Fc Tag, Gly 58 - Asp 400 (Accession # Q9NQS3) was produced in human 293 cells (HEK293).

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• ProteinLength: Gly 58 - Asp 400
• Description: Poliovirus receptor-related 3 (PVRL3), also known as nectin-3 and CD113, is a human protein of the immunoglobulin superfamily which forms part of adherens junctions. Nectins are immunoglobulin-like adhesion molecules that interact with afadin (AF6; MIM 159559). Afadin is an actin filament-binding protein that connects nectins to the actin cytoskeleton. The nectin-afadin system organizes adherens junctions cooperatively with the cadherin system in epithelial cells. PVRL3 plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Furthermore, PVRL3 induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation.
• Predicted N Terminal: Gly 58
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5, 10% trehalose.
• Molecular Mass: Human Nectin-3, Fc Tag is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 64.3 kDa. The reducing (R) protein migrates as 80-100 kDa in SDS-PAGE due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Shipping: Powder,RT

Gene Information

• Gene Name: NECTIN3 nectin cell adhesion molecule 3 [ Homo sapiens (human) ]
• Official Symbol: NECTIN3
• Synonyms: PPR3; PRR3; CD113; PVRL3; PVRR3; CDW113; NECTIN-3; nectin 3; poliovirus receptor-related 3; poliovirus receptor-related protein 3; nectin-3
• Gene ID: 25945
• mRNA Refseq: NM_001243286.1
• Protein Refseq: NP_001230215.1
• UniProt ID: Q9NQS3