Recombinant Human NCR3LG1 protein, Avi & His-tagged, Biotinylated

• Cat.No.: NCR3LG1-1290H
• Product Overview: Biotinylated Human B7-H6, His Tag is expressed from human 293 cells (HEK293). It contains AA Asp 25 - Ser 262 (Accession # Q68D85-1). Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Asp 25 - Ser 262
• Description: The B7 family of genes is essential in the regulation of the adaptive immune system. one of which is the recently discovered B7H6. Humans and rats have a single B7H6 gene; however, many B7H6 genes were detected in a single large cluster in the Xenopus genome. Chimeric antigen receptor (CAR) T-cell therapies have demonstrated durable and potentially curative therapeutic efficacy against B-cell leukemia in clinical trials. In this study, B7H6, a ligand for the NK cell activating receptor NKp30, was targeted to create a CAR that targets multiple tumor types. B7H6 is expressed on various primary human tumors, including leukemia, lymphoma and gastrointestinal stromal tumors, but it is not constitutively expressed on normal tissues.
• Predicted N Terminal: Asp 25
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 30.4 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  Please avoid repeated freeze-thaw cycles.
  No activity loss is observed after storage at:
  4-8°C for 12 months in lyophilized state;
  -70°C for 3 months under sterile conditions after reconstitution
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: NCR3LG1 natural killer cell cytotoxicity receptor 3 ligand 1 [ Homo sapiens (human) ]
• Official Symbol: NCR3LG1
• Synonyms: B7H6; B7-H6; DKFZp686O24166; B7 homolog 6; putative Ig-like domain-containing protein DKFZp686O24166/DKFZp686I21167; natural cytotoxicity triggering receptor 3 ligand 1
• Gene ID: 374383
• mRNA Refseq: NM_001202439.2
• Protein Refseq: NP_001189368.1
• UniProt ID: Q68D85