Recombinant Human NCR3 protein, His-Avi-tagged, Biotinylated

• Cat.No.: NCR3-197H
• Product Overview: Biotinylated Recombinant Human NCR3 protein(AAH52582)(Leu19-Thr138), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Leu19-Thr138
• Tag: C-His-Avi
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human NKp30, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NKp30 Antibody, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA (QC Test).
• Molecular Mass: The protein has a predicted MW of 20 kDa. Due to glycosylation, the protein migrates to 26-45 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: NCR3 natural cytotoxicity triggering receptor 3 [ Homo sapiens ]
• Official Symbol: NCR3
• Synonyms: NCR3; natural cytotoxicity triggering receptor 3; LY117, lymphocyte antigen 117; 1C7; CD337; NKp30; NK-p30; lymphocyte antigen 117; activating NK-A1 receptor; activating natural killer receptor p30; natural killer cell p30-related protein; MALS; LY117
• Gene ID: 259197
• mRNA Refseq: NM_001145466
• Protein Refseq: NP_001138938
• MIM: 611550
• UniProt ID: O14931