Recombinant Human NAGLU protein, His & T7-tagged

• Cat.No.: NAGLU-7905H
• Product Overview: Recombinant Human NAGLU aa. (Val485~Trp743 (Accession # P54802)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes an enzyme that degrades heparan sulfate by hydrolysis of terminal N-acetyl-D-glucosamine residues in N-acetyl-alpha-D-glucosaminides. Defects in this gene are the cause of mucopolysaccharidosis type IIIB (MPS-IIIB), also known as Sanfilippo syndrome B. This disease is characterized by the lysosomal accumulation and urinary excretion of heparan sulfate.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 33.3kDa
• Protein length: Val485~Trp743
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 7.8.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in ddH2O.

Gene Information

• Gene Name: NAGLU N-acetyl-alpha-glucosaminidase [ Homo sapiens (human) ]
• Official Symbol: NAGLU
• Synonyms: NAGLU; N-acetyl-alpha-glucosaminidase; NAG; CMT2V; MPS3B; UFHSD; MPS-IIIB; alpha-N-acetylglucosaminidase; N-acetylglucosaminidase, alpha; testicular tissue protein Li 18
• Gene ID: 4669
• mRNA Refseq: NM_000263.3
• Protein Refseq: NP_000254.2
• UniProt ID: P54802