Recombinant Human MSLN protein, His-Avi-tagged

Cat.No. : MSLN-7818H
Product Overview : Recombinant Human MSLN protein(Q13421-2)(Glu296-Gly580), fused with C-terminal His and Avi tag, was expressed in HEK293.
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Source : HEK293
Species : Human
Tag : C-His-Avi
Protein length : Glu296-Gly580
Form : Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized Human MSLN, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA.
Molecular Mass : The protein has a predicted MW of 35.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Endotoxin : Less than 1EU per μg by the LAL method.
Purity : > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Storage : Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Reconstitution : Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Gene Name MSLN mesothelin [ Homo sapiens ]
Official Symbol MSLN
Synonyms MSLN; mesothelin; CAK1; MPF; CAK1 antigen; megakaryocyte potentiating factor; soluble MPF mesothelin related protein; pre-pro-megakaryocyte-potentiating factor; SMRP;
Gene ID 10232
mRNA Refseq NM_001177355
Protein Refseq NP_001170826
MIM 601051
UniProt ID Q13421

Not For Human Consumption!

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