Recombinant Human MSLN protein, hFc-tagged

Source :	HEK293
Species :	Human
Tag :	C-hFc
Protein length :	Glu296-Gly580
Form :	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Bio-activity :	Immobilized Human CA125, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human MSLN, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA.
Molecular Mass :	The protein has a predicted MW of 59 kDa. Due to glycosylation, the protein migrates to 68-78 kDa based on Tris-Bis PAGE result.
Endotoxin :	Less than 1EU per μg by the LAL method.
Purity :	> 95% as determined by Tris-Bis PAGE
Storage :	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Reconstitution :	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Name	MSLN mesothelin [ Homo sapiens ]
Official Symbol	MSLN
Synonyms	MSLN; mesothelin; CAK1; MPF; CAK1 antigen; megakaryocyte potentiating factor; soluble MPF mesothelin related protein; pre-pro-megakaryocyte-potentiating factor; SMRP;
Gene ID	10232
mRNA Refseq	NM_001177355
Protein Refseq	NP_001170826
MIM	601051
UniProt ID	Q13421