Recombinant Human Mononucleosomes (H3.3G34W), Biotinylated

• Cat.No.: NUC-051
• Product Overview: Recombinant human histone H3.3 (H3F3A, H3.3A, H3F3, accession P84243) containing a methionine at position 4, expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Recombinant human histone H3.3 (H3F3A, H3.3A, H3F3, accession P84243) containing a methionine at position 4, expressed in E. coli. Histone H3 is one of the four proteins that are present in the nucleosome, the basic repeating unit subunit of chromatin, consisting of 147 base pairs of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). H3.3 is a histone variant, a non-allelelic replacement histone found in regions of high chromatin turnover outside of S-phase (e.g. at actively transcribed genes). The substitution of W for G at position 34 is associated with pediatric diffuse intrinsic pontine gliomas (DIPGs) with and diminished levels of H3K36 methylation. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5' biotin-TEG group.
• Form: Mononucleosomes (H3.3G34W), Recombinant Human (27.3 µg protein weight, 50 µg DNA+protein) in 10 mM Tris HCl, pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol.
• Molecular Mass: 200,621 Da
• Applications: Mononucleosomes (H3.3G34W), Recombinant Human are highly purified and suitable for use as substrates in enzyme screening assays, structural studies, or effector protein binding experiments. Mononucleosomes (H3.3G34W), Recombinant Human do not contain free DNA which could alter assayed activities.
• Storage: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.