Recombinant Human Mononucleosomes (H3.3G34R), Biotinylated

• Cat.No.: NUC-049
• Product Overview: Recombinant human histone H3.3 (H3F3A, H3.3A, H3F3, accession P84243) containing an arginine at position 34, expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Recombinant human histone H3.3 (H3F3A, H3.3A, H3F3, accession P84243) containing an arginine at position 34, expressed in E. coli. Histone H3 is one of the four proteins that are present in the nucleosome, the basic repeating unit subunit of chromatin, consisting of 147 base pairs of DNA containing a 5' biotin-TEG group wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). H3.3 is a histone variant, a non-allelelic replacement histone found in regions of high chromatin turnover outside of S-phase (e.g. at actively transcribed genes).
• Form: Mononucleosomes (H3.3G34R), Recombinant Human (27.3 µg protein weight, 50 µg DNA+protein) in 10mM Tris HCl, pH 7.5, 25mM NaCl, 1 mM EDTA, 2mM DTT, 20% glycerol.
• Molecular Mass: 199,905.2 Da
• Applications: Mononucleosomes (H3.3G34R), Recombinant Human are highly purified and are suitable for use as substrates in enzyme screening assays or for nucleosome binding experiments. The absence of post-translational histone modifications makes them ideal for conducting enzyme activity and screening assays. Mononucleosomes (H3.3G34R), Recombinant Human do not contain free DNA which could alter assayed activities.
• Storage: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.