Recombinant Human MMP2 protein, His-tagged
Cat.No. : | MMP2-4564H |
Product Overview : | Recombinant Human MMP2 protein(Tyr110~Cys660), fused with N-terminal His tag, was expressed in E. coli. |
- Specification
- Gene Information
- Related Products
- Download
Species : | Human |
Source : | E. coli |
Tag : | His |
Protein Length : | 110-660 aa |
Form : | 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% sarcosyl, 5% trehalose. |
Bio-activity : | Mechanism: MMP2 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family. It is a gelatinase A, 72kDa type IV collagenase which can hydrolyze gelatin under certain conditions. Gelatin zymography is mainly used for the detection of the gelatinases, MMP-2 and MMP-9 and It is extremely sensitive because levels of 10pg of MMP-2 can already be detected. Briefly, various concentrations of MMP2 (500ng, 125ng, 63ng, 32ng, 16ng) were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphate-polyacrylamide gel (SDS-PAGE; 10% gels) containing gelatin (1mg/mL) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP2 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel. In this experiment we use heat-denatured MMP2 protein as negative control, and blood sample as positive control. |
Molecular Mass : | 63.3kDa |
Purity : | >98% |
Storage : | Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month, -20°C for 12 months. Aliquot and store at -80°C for 12 month. |
Reconstitution : | Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
Gene Name | MMP2 matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) [ Homo sapiens ] |
Official Symbol | MMP2 |
Synonyms | MMP2; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); CLG4, CLG4A, matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IV collagenase) , matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); 72 kDa type IV collagenase; TBE 1; MMP-2; gelatinase A; 72 kDa gelatinase; collagenase type IV-A; neutrophil gelatinase; matrix metalloproteinase-2; matrix metalloproteinase-II; CLG4; MONA; CLG4A; TBE-1; MMP-II; |
mRNA Refseq | NM_001127891 |
Protein Refseq | NP_001121363 |
MIM | 120360 |
UniProt ID | P08253 |
Gene ID | 4313 |
◆ Recombinant Proteins | ||
MMP2-1611HFL | Recombinant Full Length Human MMP2 Protein, C-Flag-tagged | +Inquiry |
Mmp2-246R | Recombinant Rat Mmp2 protein, His-tagged | +Inquiry |
MMP2-29435TH | Recombinant Human MMP2, His-tagged | +Inquiry |
MMP2-169H | Recombinant Human MMP2 | +Inquiry |
Mmp2-5467R | Recombinant Rat Mmp2 protein, His-tagged | +Inquiry |
◆ Native Proteins | ||
MMP2-46H | Native Human MMP-2 | +Inquiry |
MMP2-47H | Native Human MMP-2/TIMP-2 Complex | +Inquiry |
MMP2-29475TH | Native Human MMP2 | +Inquiry |
MMP2-8455M | Native Mouse MMP2 | +Inquiry |
◆ Cell & Tissue Lysates | ||
MMP2-2380HCL | Recombinant Human MMP2 cell lysate | +Inquiry |
Not For Human Consumption!
Inquiry
- Reviews
- Q&As
Ask a Question for All MMP2 Products
Required fields are marked with *
My Review for All MMP2 Products
Required fields are marked with *
0
Inquiry Basket