Recombinant Human Mitogen-Activated Protein Kinase Kinase Kinase 11, GST-Tagged

• Cat.No.: MAP3K11-1039H
• Product Overview: Recombinant human MAP3K11 (1-488) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 1-488 a.a.
• Description: MAP3K11 or Mixed-Lineage Kinase 3 is a mitogen-activated protein kinase kinase kinase capable of preferentially activating MAPK8/JNK kinase and functions as a positive regulator of JNK signaling pathway. MAP3K11 can directly phosphorylate and activate IkappaB kinase α and β, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. MAP3K11 is a signal-integrating kinase with conventional MAP3K catalytic activity and additional noncatalytic functions that contribute to RAF/ERK signalling.
• Applications: Kinase Assay, Western Blot
• Molecular Weight: 83 kDa
• Expression System: Sf9 insect cells using baculovirus.
• Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10mM glutathione, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
• Specific Activity: 9.5 nmol/min/mg
• Purity: > 85 %
• Concentration: 0.1 ug/ul
• Sequences: 1-488
• Storage: Store product at -70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: CDC42 signaling events; IFN-gamma pathway; Insulin Signaling; MAPK signaling pathway; RAC1 signaling pathway; Reelin signaling pathway; superpathway of gluconate degradation

Gene Information

• Gene Name: MAP3K11 mitogen-activated protein kinase kinase kinase 11 [ Homo sapiens ]
• Official Symbol: MAP3K11
• Synonyms: MAP3K11; MLK3; MGC17114; PTK1; SPRK; MLK-3; MEKK11; mitogen-activated protein kinase kinase kinase 11; mixed lineage kinase 3; protein-tyrosine kinase PTK1; SH3 domain-containing proline-rich kinase; OTTHUMP00000233268; src-homology 3 domain-containing proline-rich kinase; OTTHUMP00000233249
• Gene ID: 4296
• mRNA Refseq: NM_002419
• Protein Refseq: NP_002410
• MIM: 600050
• UniProt ID: Q16584
• Chromosome Location: 11q13.1-q13.3
• Function: ATP binding; JUN kinase kinase kinase activity; Rac GTPase binding; identical protein binding; mitogen-activated protein kinase kinase binding; mitogen-activated protein kinase kinase kinase binding; nucleotide binding; protein binding; protein homodimerization activity; protein kinase activity; protein serine/threonine kinase activity; protein tyrosine kinase activity