Recombinant Human Mitogen-Activated Protein Kinase kinase 4, His-tagged

• Cat.No.: MAP2K4-597H
• Product Overview: Recombinant human MAP2K4 protein, was expressed as 6 his-tagged fusion protein by E. coli and purified by Ni-sepharose.

Specification

• Species: Human
• Source: Human
• Tag: His
• Description: MAP2K4 encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. MAP2K4 is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK1. MAP2K4 is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver.
• Expression Vector: PET28a
• Form: The puri?ed protein was resolved in 1 M PBS (58 mM Na2HPO4,17 mM NaH2PO4, 68 mM NaCl, pH 8.) added with 300 mM Imidazole and 0.7% Sarcosyl, 15% glycerol.
• Buffer: PBS based buffer
• Sequence: 50-399 amino acids
• Molecular Mass: 44 kDa
• Storage: The protein is stored in PBS bu?er at -20°C. Repeated freeze-thaw cycles should be avoided.

Gene Information

• Gene Name: MAP2K4 mitogen-activated protein kinase kinase 4 [ Homo sapiens ]
• Official Symbol: MAP2K4
• Synonyms: MAP2K4; mitogen-activated protein kinase kinase 4; SERK1; SEK1; SAPK/ERK kinase 1; PRKMK4; MKK4; MEK4; MAPKK4; MAPK/ERK kinase 4; MAP kinase kinase 4; JNKK1; JNKK; JNK-activating kinase 1; JNK-activated kinase 1; EC 2.7.12.2; dual specificity mitogen-activated protein kinase kinase 4; Dual specificity mitogen-activated protein kinase kinase 4; c-Jun N-terminal kinase kinase 1
• Gene ID: 6416
• mRNA Refseq: NM_003010
• Protein Refseq: NP_003001
• MIM: 601335
• UniProt ID: P45985
• Chromosome Location: 17p11.2
• Pathway: Activated TLR4 signalling; Apoptosis; CD40/CD40L signaling; CDC42 signaling events; Cellular roles of Anthrax toxin; Ceramide signaling pathway; Chagas disease (American trypanosomiasis); Cytokine Signaling in Immune system; Ephrin B reverse signaling; Epithelial cell signaling in Helicobacter pylori infection; ErbB signaling pathway; ErbB1 downstream signaling; FAS pathway and Stress induction of HSP regulation; Fc epsilon RI signaling pathway; GnRH signaling pathway
• Function: ATP binding; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; protein tyrosine kinase activity