Recombinant Human Mitogen-activated Protein Kinase-Activated Protein Kinase 3, GST-tagged

• Cat.No.: MAPKAPK3-1055H
• Product Overview: Recombinant full-length human MAPKAPK3 was expressed in E. coli cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Description: MAPKAPK3 is closely related to MAPKAPK2 sharing 72% nucleotide and 75% amino acid identity. MAPKAPK3 is activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of MAPKAPK3 as an integrative element of signaling in both mitogen and stress responses.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 69 kDa
• Expression System: E. coli
• Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
• Purity: > 90%
• Concentration: 0.2 ug/ul
• Sequences: Full-length
• Storage: Store product at -70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Activated TLR4 signalling; FAS pathway and Stress induction of HSP regulation; Immune System; MAP kinase activation in TLR cascade; MAPK signaling pathway; MyD88 cascade initiated on plasma membrane; MyD88 dependent cascade initiated on endosom; MyD88:Mal cascade initiated on plasma membrane; NFkB and MAP kinases activation mediated by TLR4 signaling repertoire; NGF signalling via TRKA from the plasma membrane; Signaling mediated by p38-alpha and p38-beta; Signalling by NGF

Gene Information

• Gene Name: MAPKAPK3 mitogen-activated protein kinase-activated protein kinase 3 [ Homo sapiens ]
• Official Symbol: MAPKAPK3
• Synonyms: MAPKAPK3; mitogen-activated protein kinase-activated protein kinase 3; 3PK; MAPKAP3; MAPKAPK-3; MAP kinase-activated protein kinase 3; MAPKAP kinase 3; OTTHUMP00000210805; OTTHUMP00000210806; OTTHUMP00000210807; chromosome 3p kinase; MAPK-activated protein kinase 3; EC 2.7.11.1
• Gene ID: 7867
• mRNA Refseq: NM_004635
• Protein Refseq: NP_004626
• MIM: 602130
• UniProt ID: Q16644
• Chromosome Location: 3p21.3
• Function: ATP binding; MAP kinase kinase activity; nucleotide binding; protein binding; protein serine/threonine kinase activity