Recombinant Human mitochondrial ribosome recycling factor, His-tagged

• Cat.No.: MRRF-387H
• Product Overview: MRRF, 56-262aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 56-262 a.a.
• Description: MRRF binds to the large ribosomal subunit in the cleft that contains the peptidyl transferase center. This protein is responsible for the release of ribosome from messenger RNA at the termination of protein biosynthesis. Also, it may increase the efficiency of translation by recycling ribosome from one round of translation to another. Recombinant human MRRF protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 30% glycerol,2mM DTT
• Molecular Mass: 25.1 kDa (228aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MATKKAKAKG KGQSQTRVNI NAALVEDIIN LEEVNEEMKS VIEALKDNFN KTLNIRTSPG SLDKIAVVTA DGKLALNQIS QISMKSPQLI LVNMASFPEC TAAAIKAIRE SGMNLNPEVE GTLIRVPIPQ VTREHREMLV KLAKQNTNKA KDSLRKVRTN SMNKLKKSKD TVSEDTIRLI EKQISQMADD TVAELDRHLA VKTKELLG
• Purity: >95% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: MRRF mitochondrial ribosome recycling factor [ Homo sapiens ]
• Official Symbol: MRRF
• Synonyms: MRRF; mitochondrial ribosome recycling factor; ribosome-recycling factor, mitochondrial; RRF; ribosome-releasing factor, mitochondrial; MRFF; MTRRF
• Gene ID: 92399
• mRNA Refseq: NM_001173512
• Protein Refseq: NP_001166983
• MIM: 604602
• UniProt ID: Q96E11
• Chromosome Location: 9q32-q34.1