Recombinant Human mitochondrial ribosomal protein L1, His-tagged

• Cat.No.: MRPL1-537H
• Product Overview: MRPL1, 51-325aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 51-325 a.a.
• Description: 39S ribosomal protein L1, mitochondrial precursor, also known as MRPL1, is a 338 amino acid mitochondrial protein that exists as a component of the 39S ribosomal subunit and works in conjunction with other MRPs to mediate protein synthesis. Mitochondrial ribosomes consist of a large 39S subunit and a small 28S subunit, both of which are comprised of multiple mitochondrial ribosomal proteins (MRPs) that are encoded by nuclear genes and are essential for protein synthesis within mitochondria. MRPL1 exists as two isoforms produced by alternative splicing. Isoform one of MRPL1 is ubiquitously expressed while isoform two is specifically expressed in heart. Recombinant human MRPL1 proteinto His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 30% glycerol, 1mM DTT
• Molecular Mass: 33.8kDa (298aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSKKTKKGA KEKTPDEKKD EIEKIKAYPY MEGEPEDDVY LKRLYPRQIY EVEKAVHLLK KFQILDFTSP KQSVYLDLTL DMALGKKKNV EPFTSVLSLP YPFASEINKV AVFTENASEV KIAEENGAAF AGGTSLIQKI WDDEIVADFY VAVPEIMPEL NRLRKKLNKK YPKLSRNSIG RDIPKMLELF KNGHEIKVDE ERENFLQTKI ATLDMSSDQI AANLQAVINE VCRHRPLNLG PFVVRAFLRS STSEGLLLKI DPLLPKEVKN EESEKEDA
• Purity: >80% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: MRPL1 mitochondrial ribosomal protein L1 [ Homo sapiens ]
• Official Symbol: MRPL1
• Synonyms: MRPL1; mitochondrial ribosomal protein L1; 39S ribosomal protein L1, mitochondrial; BM022; L1MT; MRP-L1; FLJ96680
• Gene ID: 65008
• mRNA Refseq: NM_020236
• Protein Refseq: NP_064621
• MIM: 611821
• UniProt ID: Q9BYD6
• Chromosome Location: 4q21.1
• Function: RNA binding; structural constituent of ribosome