Recombinant Human MET protein, His-tagged, Site-Specific AF 488-Labeled

• Cat.No.: MET-6744H
• Product Overview: Recombinant Human MET protein(NP_000236.2) (Met1-Thr932) was expressed in HEK293 with a polyhistidine tag at the C-terminus. The protein is site-specifically labeled with AF 488 (Excitation Max. = 495 nm, Emission Max. = 519 nm).

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Met1-Thr932
• Form: This product is Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: 1. Flow cytometric analysis of anti-c-MET CAR expression. 293 cells were lentivirally transduced with anti-c-MET CAR. Flow cytometric analysis was performed with a negative control protein and AF 488-conjugated human c-MET protein, respectively. Non-transduced 293 cells were used as a control (left).
  2. Binding activity of AF 488-conjugated c-MET protein to PBMC cells. PBMC cells were stained with anti-CD3 antibody and AF 488-conjugated human c-MET protein and detected by flow cytometry. PBMC cells stained with anti-CD3 antibody were used as a control (left).
• Molecular Mass: The recombinant Human MET consists of AA Met1-Thr932 with a polyhistidine tag at the C-terminus. It has a predicted molecular mass of 105.1 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: ≥ 90 % as determined by SDS-PAGE.
• Storage: Twelve months from date of receipt at -20°C to -70°C in lyophilized form and 3 months at -70°C under sterile conditions after reconstitution. Protect from prolonged exposure to light and avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: MET met proto-oncogene (hepatocyte growth factor receptor) [ Homo sapiens ]
• Official Symbol: MET
• Synonyms: MET; met proto-oncogene (hepatocyte growth factor receptor); hepatocyte growth factor receptor; HGFR; RCCP2; SF receptor; HGF receptor; oncogene MET; HGF/SF receptor; proto-oncogene c-Met; scatter factor receptor; tyrosine-protein kinase Met; met proto-oncogene tyrosine kinase; AUTS9; c-Met
• Gene ID: 4233
• mRNA Refseq: NM_000245
• Protein Refseq: NP_000236
• MIM: 164860
• UniProt ID: P08581