| Species : |
Human |
| Source : |
Human |
| Tag : |
GST |
| Protein Length : |
202-end a.a. |
| Description : |
MDM2 is a nuclear phosphoprotein that binds and inhibits transactivation by p53, as part of an autoregulatory negative feedback loop (1). Overexpression of the MDM2 gene product can lead to excessive inactivation of p53 thereby diminishing its tumor suppressor function. The inactivation of p53 is mediated by the E3 ubiquitin ligase activity of MDM2 which targets p53 for proteasomal degradation. MDM2 also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5 (2). Amplification of MDM2 is frequently observed in human sarcomas and this is consistent with the hypothesis that MDM2 binds to p53 which then leads to escape from p53-regulated growth control. |
| Applications : |
Western Blot |
| Molecular Weight : |
~84 kDa |
| Purity : |
>75% |
| Concentration : |
0.2ug/ul |
| Form : |
Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25%glycerol. |
| Storage : |
Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
| Pathways : |
AKT phosphorylates targets in the cytosol; Bladder cancer; Androgen Receptor Signaling Pathway; Cell Cycle Checkpoints; Apoptosis; Cell cycle; Aurora A signaling; Chronic myeloid leukemia; Direct p53 effectors; Endocytosis; Downstream signaling of activated FGFR; ErbB signaling pathway; G1 to S cell cycle control; G1/S DNA Damage Checkpoints; Glioma; Glucocorticoid receptor regulatory network; Glutamate Binding, Activation of AMPA Receptors and Synaptic Plasticity; Melanoma; NGF signalling via TRKA from the plasma membrane; Neuroransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell; PI-3K cascade; PI3K/AKT activation; Signaling by FGFR; PIP3 activates AKT signaling; Regulation of retinoblastoma protein; Sumoylation by RanBP2 regulates transcriptional repression |
