Recombinant Human MCL1, His-tagged

• Cat.No.: MCL1-781H
• Product Overview: Recombinant human MCL1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol, 2mM DTT
• Molecular Mass: 37.2 kDa (347aa), confirmed by MALDI-TOF
• Protein length: 1-327aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH PDSRRVARPP PIGAEVPDVT ATPARLLFFA PTRRAAPLEE MEAPAADAIM SPEEELDGYE PEPLGKRPAV LPLLELVGES GNNTSTDGSL MFGLKRNAVI GLNLYCGGAG LGAGSGGATR PGGRLLATEK EASARREIGG GEAGAVIGGS AGASPPSTLT PSTPPPAEEE EDELYRQSLE IISRYLREQA TGAKDTKPMG RSGATSRKAL ETLRRVGDGV QRNHETAFQG MLRKLDIKNE DDVKSLSRVM IHVFSDGVTN WGRIVTLISF GAFVAKHLKT INQESCIEPL AESITDVLVR TKRDWLVKQR GWDGFVEFFH VEDLEGG
• Purity: > 90% by SDS - PAGE
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)
• Publications:
  Mcl-1 Interacts with Akt to Promote Lung Cancer Progression (2019)
  Mcl-1 Interacts with Akt to Promote Lung Cancer Progression (2019)

Gene Information

• Gene Name: MCL1 myeloid cell leukemia 1 [ Homo sapiens (human) ]
• Official Symbol: MCL1
• Synonyms: MCL1; myeloid cell leukemia sequence 1 (BCL2-related); induced myeloid leukemia cell differentiation protein Mcl-1; BCL2L3; Mcl 1; bcl-2-like protein 3; myeloid cell leukemia ES; bcl-2-related protein EAT/mcl1; TM; EAT; MCL1L; MCL1S; Mcl-1; MCL1-ES; bcl2-L-3; mcl1/EAT; MGC1839; MGC104264
• Gene ID: 4170
• mRNA Refseq: NM_021960
• Protein Refseq: NP_068779
• MIM: 159552
• UniProt ID: Q07820
• Chromosome Location: 1q21
• Pathway: Apoptosis Modulation and Signaling; E2F transcription factor network; IL-7 Signaling Pathway
• Function: BH3 domain binding; protein channel activity; protein heterodimerization activity