Recombinant Human matrix metallopeptidase 13 (collagenase 3) Protein, His tagged

• Cat.No.: MMP13-038H
• Product Overview: The sequence (Accession # 1EUB_A) corresponding to the catalytic domain (aa 104-274 aa) of human MMP-13 was expressed with 6×His tag in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 104-274aa
• Description: This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. Mutations in this gene are associated with metaphyseal anadysplasia. This gene is part of a cluster of MMP genes on chromosome 11.
• Tag: 6×His
• Form: Solution
• Molecular Mass: 20,4 kDa
• Purity: ≥95% by SDS-PAGE
• Applications: FRET; Protease activity
• Usage: Research use
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 10 μg/ml
• Storage Buffer: 0.1M Tris, pH 7.5, 0.1M NaCl, 10 mM CaCl2, 0.05% Brij 35, 2mM sodium azide, 1 mg/mL BSA.
• References: 1. Freije, J. et al. J. Biol .Chem. 269, 16766 (1994).

Gene Information

• Gene Name: MMP13 matrix metallopeptidase 13 (collagenase 3) [ Homo sapiens (human) ]
• Official Symbol: MMP13
• Synonyms: MMP13; matrix metallopeptidase 13 (collagenase 3); matrix metalloproteinase 13 (collagenase 3); collagenase 3; CLG3; MMP-13; MANDP1
• Gene ID: 4322
• mRNA Refseq: NM_002427
• Protein Refseq: NP_002418
• MIM: 600108
• UniProt ID: P45452