Recombinant Human MAPT protein, His-tagged

• Cat.No.: MAPT-3533H
• Product Overview: Recombinant Human MAPT protein(P10636)(1-441aa), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: N-His
• Protein length: 1-441aa
• Form: Lyophilized from a 0.2 um filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human MAPT at 2 μg/ml can bind Anti-MAPT recombinant antibody, the EC50 is 4.547-6.284 ng/mL.
• Molecular Mass: 49.5 kDa
• AASequence: MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL
• Endotoxin: Less than 1.0 EU/ug as determined by LAL method.
• Purity: Greater than 95% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C.

Gene Information

• Gene Name: MAPT microtubule-associated protein tau [ Homo sapiens ]
• Official Symbol: MAPT
• Synonyms: MAPT; microtubule-associated protein tau; DDPAC, MAPTL; FLJ31424; FTDP 17; G protein beta1/gamma2 subunit interacting factor 1; MGC138549; microtubule associated protein tau; isoform 4; MSTD; MTBT1; MTBT2; PPND; tau; TAU; PHF-tau; paired helical filament-tau; neurofibrillary tangle protein; microtubule-associated protein tau, isoform 4; G protein beta1/gamma2 subunit-interacting factor 1; DDPAC; MAPTL; FTDP-17
• Gene ID: 4137
• mRNA Refseq: NM_001123066
• Protein Refseq: NP_001116538
• MIM: 157140
• UniProt ID: P10636