Recombinant Human MAPK3

• Cat.No.: MAPK3-34H
• Product Overview: Recombinant Human ERK1/MAPK3 is a highly active form produced by phosphorylation of the purified ERK1/MAPK3 in vitro with MEK1 is a non-glycosylated polypeptide having a molecular mass of 43.6 kDa. ERK1/MAPK3 is purified by proprietary chromatographic techniques. It is producted inEscherichia Coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Extracellular signal-regulated kinases (ERKs) or classical MAP kinases are widely expressed protein kinase intracellular signaling molecules which are involved in functions including the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric G protein-coupled receptors, transforming agents, and carcinogens, activate the ERK pathway. The term, "extracellular signal-regulated kinases", is sometimes used as a synonym for mitogen-activated protein kinase (MAPK), but has more recently been adopted for a specific subset of the mammalian MAPK family. In the MAPK/ERK pathway, Ras activates c-Raf, followed by MEK and then MAPK1/2 (below).
• Physical Appearance: Sterile Filtered clear solution.
• Unit Definition: No protease activity detectable, specific activity > 15.000 U*/mg (*1 U = 1 pmol/min transferred to myelin basic protein at 30 degree C).
• Purity: Greater than 95% as determined by SDS-PAGE.
• Formulation: ERK1/MAPK3 is supplied as 0.15mg/ml containing 50mM Tris-HCl, 150mM NaCl, 1mM DTT, 50% glycerol, pH 8.5.
• Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

Gene Information

• Gene Name: MAPK3 mitogen-activated protein kinase 3 [ Homo sapiens ]
• Synonyms: MAPK3; mitogen-activated protein kinase 3; ERK1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; MGC20180;EC2.7.11.24; Extracellular signal-regulated kinase 1; Insulin-stimulated MAP2 kinase; MAP kinase 1; Microtubule-associated protein 2 kinas; OTTHUMP00000174538; OTTHUMP00000174540; Microtubule-associated protein 2 kinase; mitogen-activated protein kinase 3
• Gene ID: 5595
• mRNA Refseq: NM_001040056
• Protein Refseq: NP_001035145
• UniProt ID: P27361
• MIM: 601795
• Pathway: Acute myeloid leukemia; Adherens junction; Alzheimer"s disease; Alzheimer"s disease; B cell receptor signaling pathway; Bladder cancer; Chronic myeloid leukemia; Chemokine signaling pathway; Colorectal cancer; Dorso-ventral axis formation; Endometrial cancer; ErbB signaling pathway; Fc epsilon RI signaling pathway; Fc gamma R-mediated phagocytosis; Gap junction; Glioma; GnRH signaling pathway; Insulin signaling pathway; Long-term potentiation; Long-term depression; MAPK signaling pathway; Melanogenesis; Melanoma; Natural killer cell mediated cytotoxicity; Neurotrophin signaling pathway; Non-small cell lung cancer; Pancreatic cancer; Pathways in cancer; Prion diseases; Regulation of actin cytoskeleton; Renal cell carcinoma; Renal cell carcinoma; T cell receptor signaling pathway; TGF-beta signaling pathway; Thyroid cancer; Toll-like receptor signaling pathway; Type II diabetes mellitus; VEGF signaling pathway; Vascular smooth muscle contraction; mTOR signaling pathway; Axon guidance; Signaling by Insulin receptor; Signaling by EGFR; Signaling by PDGF; Signalling by NGF; Transcription
• Function: ATP binding; MAP kinase activity; nucleotide binding; protein binding; protein serine/threonine kinase activity; transferase activity