Recombinant Human MAP2K2, His-tagged

• Cat.No.: MAP2K2-15860H
• Product Overview: Recombinant human MAP2K2 proetin, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-400aa
• Description: MAP2K2, also known as MEK2, belongs to the STE grouping of kinases. Both MEK2 and the related MEK1 are dualspecificity kinases, phosphorylating and activating the mitogen-activated protein kinases ERK1 and ERK2 at T and Y positions within the phosphoacceptor sequence T-E-Y. MAP2K2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol
• Molecular Mass: 46.9 kDa (424aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMLARRK PVLPALTINP TIAEGPSPTS EGASEANLVD LQKKLEELEL DEQQKKRLEA FLTQKAKVGE LKDDDFERIS ELGAGNGGVV TKVQHRPSGL IMARKLIHLE IKPAIRNQII RELQVLHECN SPYIVGFYGA FYSDGEISIC MEHMDGGSLDQVLKEAKRIP EEILGKVSIA VLRGLAYLRE KHQIMHRDVK PSNILVNSRG EIKLCDFGVS GQLIDSMANS FVGTRSYMAP ERLQGTHYSV QSDIWSMGLS LVELAVGRYP IPPPDAKELE AIFGRPVVDG EEGEPHSISP RPRPPGRPVS GHGMDSRPAM AIFELLDYIV NEPPPKLPNGVFTPDFQEFV NKCLIKNPAE RADLKMLTNH TFIKRSEVEE VDFAGWLCKT LRLNQPGTPT RTAV
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term. For long term storage, aliquot and store at at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: MAP2K2 mitogen-activated protein kinase kinase 2 [ Homo sapiens ]
• Official Symbol: MAP2K2
• Synonyms: MAP2K2; mitogen-activated protein kinase kinase 2; PRKMK2; dual specificity mitogen-activated protein kinase kinase 2; MEK2; MAPK/ERK kinase 2; MAP kinase kinase 2; ERK activator kinase 2; mitogen-activated protein kinase kinase 2, p45; MKK2; MAPKK2; FLJ26075
• Gene ID: 5605
• mRNA Refseq: NM_030662
• Protein Refseq: NP_109587
• MIM: 601263
• UniProt ID: P36507
• Chromosome Location: 19p13.3
• Pathway: ARMS-mediated activation, organism-specific biosystem; Activated TLR4 signalling, organism-specific biosystem; Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Axon guidance, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; B cell receptor signaling pathway, organism-specific biosystem
• Function: ATP binding; nucleotide binding; protein binding; protein complex scaffold; protein serine/threonine kinase activity; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity; protein tyrosine kinase activity