Recombinant Human MANF protein, His-tagged

• Cat.No.: MANF-518H
• Product Overview: Recombinant Human MANF (P55145) fused with His tag was produced in HEK293F cells.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Description: The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related.
• Form: Freeze-dried powder
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: MANF mesencephalic astrocyte derived neurotrophic factor [ Homo sapiens (human) ]
• Official Symbol: MANF
• Synonyms: MANF; mesencephalic astrocyte derived neurotrophic factor; ARP; ARMET; mesencephalic astrocyte-derived neurotrophic factor; arginine-rich, mutated in early stage tumors
• Gene ID: 7873
• mRNA Refseq: NM_006010.5
• Protein Refseq: NP_006001.4
• UniProt ID: P55145