Recombinant Human MAD2L1, His-tagged

• Cat.No.: MAD2L1 -161H
• Product Overview: Recombinant Human MAD2L1 /MAD2 Protein (rh MAD2L1 /MAD2) Met 1 - Asp 205 (Accession # NP_002349) was produced in E.coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Met1-Asp205
• Description: Mitotic arrest deficient 2-like protein 1 (MAD2L1) is also known as Mitotic spindle assembly checkpoint protein MAD2A (HsMAD2), MAD2. MAD2L1 / MAD2 belongs to the MAD2 family. MAD2L1 / MAD2 contains one HORMA domain. MAD2L1 is the component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 / MAD2 is required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Molecular Mass: rh MAD2L1 /MAD2 is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 24.3 kDa. The predicted N-terminus is Met 1. DTT-reduced Protein migrates as 24 kDa in SDS-PAGE.
• Endotoxin: Less than 1.0 EU per μg of the rh MAD2L1 /MAD2 by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
• Reconstitution: See Certificate of Analysis for reconstitution instructions and specific concentrations.

Gene Information

• Gene Name: MAD2L1 MAD2 mitotic arrest deficient-like 1 (yeast) [ Homo sapiens ]
• Official Symbol: MAD2L1
• Synonyms: MAD2L1; MAD2 mitotic arrest deficient-like 1 (yeast); MAD2 (mitotic arrest deficient, yeast, homolog) like 1; mitotic spindle assembly checkpoint protein MAD2A; HSMAD2; MAD2; MAD2-like protein 1; mitotic arrest deficient 2-like protein 1; mitotic arrest deficient, yeast, homolog-like 1; MAD2 (mitotic arrest deficient, yeast, homolog)-like 1
• Gene ID: 4085
• mRNA Refseq: NM_002358
• Protein Refseq: NP_002349
• MIM: 601467
• UniProt ID: Q13257
• Chromosome Location: 4q27
• Pathway: APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; Amplificationof signal from unattachedkinetochores via a MAD2inhibitory signal, organism-specific biosystem; Amplification of signal from the kinetochores, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle Checkpoints, organism-specific biosystem
• Function: protein binding; protein homodimerization activity