Recombinant Human LUM protein, His-tagged

• Cat.No.: LUM-32H
• Product Overview: Recombinant human LUM, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Description: LUM, also known as lumican, is a member of the class II small leucine-rich proteoglycan family. It is a major component of the cornea, dermal, and muscle connective tissues. It regulates collagenous matrix assembly as a keratan sulfate proteoglycan in the cornea and is also present in the connective tissues of other organs and embryonic corneal stroma as a glycoprotein. It may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair.
• Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
• Molecular Mass: 37.7kDa (329aa)
  40-57kDa (SDS-PAGE under reducing conditions)
• AA Sequence: ADLQYYDYDF PLSIYGQSSP NCAPECNCPE SYPSAMYCDE LKLKSVPMVP PGIKYLYLRN NQIDHIDEKA FENVTDLQWL ILDHNLLENSKIKGRVFSKL KQLKKLHINH NNLTESVGPL PKSLEDLQLT HNKITKLGSF EGLVNLTFIH LQHNRLKEDA VSAAFKGLKS LEYLDLSFNQIARLPSGLPV SLLTLYLDNN KISNIPDEYF KRFNALQYLR LSHNELADSG IPGNSFNVSS LVELDLSYNK LKNIPTVNEN LENYYLEVNQLEKFDIKSFC KILGPLSYSK IKHLRLDGNR ISETSLPPDM YECLRVANEV TLNHHHHHH
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: > 95% by SDS - PAGE
• Storage: Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20centigrade or -70centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: LUM lumican [ Homo sapiens ]
• Official Symbol: LUM
• Synonyms: LUM; lumican; LDC; lumican proteoglycan; SLRR2D; KSPG lumican; keratan sulfate proteoglycan lumican
• Gene ID: 4060
• mRNA Refseq: NM_002345
• Protein Refseq: NP_002336
• MIM: 600616
• UniProt ID: P51884
• Chromosome Location: 12q21.3-q22
• Function: collagen binding; extracellular matrix structural constituent