Recombinant Human LRRC32 protein, Fc-tagged

• Cat.No.: LRRC32-396H
• Product Overview: Recombinant Human LRRC32 protein (His20-Asn627), fused to human IgG1 Fc tag at C-terminus, was expressed in human 293 cells (HEK293).

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 608
• Description: Leucine-rich repeat protein 32 (LRRC32), also known as GARP (glycoprotein A repetitions predominant). LRRC32 expression promotes the acquisition of a Treg phenotype including reduced cellular proliferation, reduced cytokine secretion, and the capacity to suppress the proliferation of naïve T cells. LRRC32 binds directly to the TGF-beta latency associated peptide (LAP) and tethers latent TGF-beta on the surface of activated Treg cells. The presentation of TGF-beta on Tregs contributes to their ability to suppress naïve T cell proliferation.
• Form: Lyophilized from 0.22 um filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 92.4 kDa. The protein migrates as 106 KDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per ug by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: LRRC32
• Official Symbol: LRRC32
• Synonyms: LRRC32; leucine rich repeat containing 32; D11S833E, GARP, glycoprotein A repetitions predominant; leucine-rich repeat-containing protein 32; garpin; glycoprotein A repetitions predominant; GARP; D11S833E
• Gene ID: 2615
• mRNA Refseq: NM_001128922
• Protein Refseq: NP_001122394
• MIM: 137207
• UniProt ID: Q14392