Recombinant Human LRP6 protein, Mouse IgG Fc-tagged

• Cat.No.: LRP6-552H
• Product Overview: Recombinant Human LRP6 (Ala20-Pro630) protein was fused to mouse IgG2a Fc tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: LRP6 is a transmembrane low-density lipoprotein receptor that shares a similar structure with LRP5. Low-density lipoprotein receptor-related protein 6 is a key component of the LRP5/LRP6/Frizzled co-receptor group that is involved in canonical Wnt pathway. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin.
• Source: HEK293
• Species: Human
• Tag: mFC
• Predicted N Terminal: Ala20
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 95.8 kDa. The protein migrates as 55 kDa and 110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Ala20-Pro630
• Endotoxin: Less than 0.1 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: LRP6
• Official Symbol: LRP6
• Synonyms: LRP6; low density lipoprotein receptor-related protein 6; low-density lipoprotein receptor-related protein 6; LRP-6; ADCAD2; FLJ90062; FLJ90421
• Gene ID: 4040
• mRNA Refseq: NM_002336
• Protein Refseq: NP_002327
• MIM: 603507
• UniProt ID: O75581