Recombinant Human LPL protein, Flag-tagged

• Cat.No.: LPL-1015H
• Product Overview: Recombinant Human LPL(Ala28 to Gly475, variant Asn > Ser318) fused with Flag tag at N-terminal and 13 extra AA was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag
• Form: Filtered (0.4 μm) and lyophilized from 0.5 mg/ml in 20mM Tris buffer, 50mM NaCl, pH 7.5
• Molecular Mass: 51.8 kDa (calculated).
• AA Sequence: HVDYKDDDDK PAGADQRRDF IDIESKFALR TPEDTAEDTC HLIPGVAESV ATCHFNHSSK TFMVIHGWTV TGMYESWVPK LVAALYKREP DSNVIVVDWL SRAQEHYPVS AGYTKLVGQD VARFINWMEE EFNYPLDNVH LLGYSLGAHA AGIAGSLTNK KVNRITGLDP AGPNFEYAEA PSRLSPDDAD FVDVLHTFTR GSPGRSIGIQ KPVGHVDIYP NGGTFQPGCN IGEAIRVIAE RGLGDVDQLV KCSHERSIHL FIDSLLNEEN PSKAYRCSSK EAFEKGLCLS CRKNRCNNLG YEISKVRAKR SSKMYLKTRS QMPYKVFHYQ VKIHFSGTES ETHTNQAFEI SLYGTVAESE NIPFTLPEVS TNKTYSFLIY TEVDIGELLM LKLKWKSDSY FSWSDWWSSP GFAIQKIRVK AGETQKKVIF CSREKVSHLQ KGKAPAVFVK CHDKSLNKKS G
• Endotoxin: < 1.0 EU/μg
• Applications: Cell culture and/or animal studies, ELISA, Western blotting
• Storage: Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 centigrade for long term storage. Reconstituted protein can be stored at 4 centigrade for a limited period of time; it does not show any change after one week at 4 centigrade.
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Gene Information

• Gene Name: LPL lipoprotein lipase [ Homo sapiens ]
• Official Symbol: LPL
• Synonyms: LPL; lipoprotein lipase; LIPD; HDLCQ11
• Gene ID: 4023
• mRNA Refseq: NM_000237
• Protein Refseq: NP_000228
• UniProt ID: P06858
• Chromosome Location: 8p22
• Pathway: Adipogenesis, organism-specific biosystem; Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Chylomicron-mediated lipid transport, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Fatty Acid Beta Oxidation, organism-specific biosystem; Glycerolipid metabolism, organism-specific biosystem
• Function: heparin binding; hydrolase activity; lipoprotein lipase activity; lipoprotein lipase activity; lipoprotein lipase activity; phospholipase activity; protein binding; receptor binding; triglyceride binding; triglyceride lipase activity; triglyceride lipase activity