Recombinant Human LCAT protein, His-tagged

• Cat.No.: LCAT-526H
• Product Overview: Recombinant Human LCAT protein(P04180)(Phe25-Glu440), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Phe25-Glu440
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human LCAT, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LCAT Antibody, hFc Tag with the EC50 of 9.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 48.2 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
• Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: LCAT lecithin-cholesterol acyltransferase [ Homo sapiens ]
• Official Symbol: LCAT
• Synonyms: LCAT; lecithin-cholesterol acyltransferase; phosphatidylcholine-sterol acyltransferase; phospholipid-cholesterol acyltransferase
• Gene ID: 3931
• mRNA Refseq: NM_000229
• Protein Refseq: NP_000220
• MIM: 606967
• UniProt ID: P04180