Recombinant Human LAT Protein, His-tagged

• Cat.No.: LAT-335H
• Product Overview: Recombinant Human LAT(Leu25～Glu221) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: E. coli
• Species: Human
• Tag: His
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Molecular Mass: 24.9kDa
• Identity: Reconstitute in PBS or others
• Protein length: Leu25～Glu221
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in PBS or others

Gene Information

• Gene Name: LAT linker for activation of T cells [ Homo sapiens ]
• Official Symbol: LAT
• Synonyms: LAT; linker for activation of T cells; linker for activation of T-cells family member 1; LAT1; transmembrane adaptor; p36-38; 36 kDa phospho-tyrosine adapter protein; 36 kDa phospho-tyrosine adaptor protein; linker for activation of T cells, transmembrane adaptor; pp36
• Gene ID: 27040
• mRNA Refseq: NM_001014987
• Protein Refseq: NP_001014987
• MIM: 602354
• UniProt ID: O43561