Recombinant Human KLRK1 protein, hFc-Flag-tagged

• Cat.No.: KLRK1-4633H
• Product Overview: Recombinant Human KLRK1 protein(P26718)(Phe78-Val216), fused with N-terminal hFc-Flag tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&Flag
• Protein Length: Phe78-Val216
• Tag: N-hFc-Flag
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human MICA, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.81μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 43.4 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: KLRK1 killer cell lectin-like receptor subfamily K, member 1 [ Homo sapiens ]
• Official Symbol: KLRK1
• Synonyms: KLRK1; killer cell lectin-like receptor subfamily K, member 1; D12S2489E, DNA segment on chromosome 12 (unique) 2489 expressed sequence; NKG2-D type II integral membrane protein; CD314; KLR; NKG2 D; NKG2D; NK cell receptor D; NKG2-D-activating NK receptor; NKG2-D; D12S2489E; FLJ17759; FLJ75772
• Gene ID: 22914
• mRNA Refseq: NM_007360
• Protein Refseq: NP_031386
• MIM: 611817
• UniProt ID: P26718