Recombinant Human KIT v-kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog, GST-tagged

• Cat.No.: KIT-4857H
• Product Overview: Recombinant human KIT (544-end) was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 544-end a.a.
• Description: KIT is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed curbing neoplastic mast cell growth.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 73 kDa
• Expression System: Sf9 insect cells using baculovirus.
• Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM EDTOA, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
• Purity: > 85 %
• Concentration: 0.1 ug/ul
• Sequences: 544-end
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Acute myeloid leukemia; Acute myeloid leukemia; C-MYB transcription factor network; Cytokine-cytokine receptor interaction; Endocytosis; Hematopoietic cell lineage; Kit Receptor Signaling Pathway; Melanogenesis; Pathways in cancer; Signaling events mediated by Stem cell factor receptor (c-Kit)

Gene Information

• Gene Name: KIT v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog [ Homo sapiens ]
• Official Symbol: KIT
• Synonyms: KIT; PBT; SCFR; C-Kit; CD117; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; EC 2.7.10.1; piebald trait; mast/stem cell growth factor receptor; proto-oncogene tyrosine-protein kinase Kit; soluble KIT variant 1; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein; CD117 antigen; EC 2.7.10
• Gene ID: 3815
• mRNA Refseq: NM_000222
• Protein Refseq: NP_000213
• MIM: 609132
• UniProt ID: P10721
• Chromosome Location: 4q11-q12
• Function: ATP binding; cytokine binding; nucleotide binding; protein binding; protein homodimerization activity; protein tyrosine kinase activity; receptor activity; receptor signaling protein tyrosine kinase activity; stem cell factor receptor activity; transmembrane receptor protein tyrosine kinase activity

Citation

Genomic Profiling: The Strengths and Limitations of Chloroplast Genome-Based Plant Variety Authentication.

Journal: Journal of agricultural and food chemistry    PubMed ID: 32917087    Data: 2021/4/1

Authors: Doreen Teske, Alina Peters, Markus Fischer

Article Snippet:Conversely, sucrose has a high osmolality, high viscosity, 222 and can penetrate into cells, leading to incoherent gradient patterns in case of osmotically 223 sensitive components, like chloroplasts.55 Several studies have successfully applied Percoll? 224 gradients to enrich chloroplasts such as Takamatsu. et al.,15 Vieira et al.,44 Shi et al.48 and Lang 225 et al.56 226 Recently, some companies have developed special kits for the chloroplast isolation, so far they 227 have been applied only on a limited number of studies.gradients to enrich chloroplasts such as Takamatsu. et al.,15 Vieira et al.,44 Shi et al.48 and Lang 225 et al.56 226 Recently, some companies have developed special kits for the chloroplast isolation, so far they 227 have been applied only on a limited number of studies. ... Moreover, there are commercial kits 228 on the market, such as the Chloroplast Isolation Kit (Merck), Chloroplast Isolation Kit (abcam), 229 Minute? chloroplast isolation kit (invent), Chloroplast Isolation Kit (Creative BioMart) , or 230 OrgFrontier? Chloroplast Isolation Kit (BioVision).. 231 If no chloroplast isolation is to be carried out, there are alternative approaches e.g. for targeted 232 enrichment of cpDNA, such as long-range PCR of the complete chloroplast genome (multiple 233 PCRs that amplify DNA which can reach a length up to 20 kbp to cover the entire chloroplast 234 genome), target enrichment using homologous hybridization probes or restriction enzyme235 based enrichment.57,58 In addition, Kane et al. established a method to sequence whole 236 genomes, which was based on low-coverage whole genome shotgun sequencing with Page 15 of 49 ACS Paragon Plus Environment Journal of Agricultural and Food Chemistry Page 16 of 49 237 genomic DNA as product and without extensive processing.231 If no chloroplast isolation is to be carried out, there are alternative approaches e.g. for targeted 232 enrichment of cpDNA, such as long-range PCR of the complete chloroplast genome (multiple 233 PCRs that amplify DNA which can reach a length up to 20 kbp to..