Recombinant Human KISS1 protein, His & T7-tagged

• Cat.No.: KISS1-1646H
• Product Overview: Recombinant Human KISS1 aa. (Glu20~Gly138 (Accession # Q15726)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Glu20~Gly138
• Description: This gene is a metastasis suppressor gene that suppresses metastases of melanomas and breast carcinomas without affecting tumorigenicity. The encoded protein may inhibit chemotaxis and invasion and thereby attenuate metastasis in malignant melanomas. Studies suggest a putative role in the regulation of events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A protein product of this gene, kisspeptin, stimulates gonadotropin-releasing hormone (GnRH)-induced gonadotropin secretion and regulates the pubertal activation of GnRH nuerons. A polymorphism in the terminal exon of this mRNA results in two protein isoforms. An adenosine present at the polymorphic site represents the third position in a stop codon. When the adenosine is absent, a downstream stop codon is utilized and the encoded protein extends for an additional seven amino acid residues.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 16.2kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.
• Isoelectric Point: 10.5

Gene Information

• Gene Name: KISS1 KiSS-1 metastasis-suppressor [ Homo sapiens (human) ]
• Official Symbol: KISS1
• Synonyms: KISS1; KiSS-1 metastasis-suppressor; HH13; KiSS-1; metastasis-suppressor KiSS-1; kisspeptin-1; malignant melanoma metastasis-suppressor; metastin; prepro-kisspeptin
• Gene ID: 3814
• mRNA Refseq: NM_002256.3
• Protein Refseq: NP_002247.3
• UniProt ID: Q15726