Recombinant Human kinase suppressor of ras 1 Protein, His tagged

• Cat.No.: KSR1-008H
• Product Overview: Recombinant human KSR1 protein (Q8IVT5) (Glu 27-Gly 172) with a His tag at the C-terminus was expressed in E. coli.

Specification

• Description: Part of a multiprotein signaling complex which promotes phosphorylation of Raf family members and activation of downstream MAP kinases. Independently of its kinase activity, acts as MAP2K1/MEK1 and MAP2K2/MEK2-dependent allosteric activator of BRAF; upon binding to MAP2K1/MEK1 or MAP2K2/MEK2, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1 and/or MAP2K2/MEK2. Promotes activation of MAPK1 and/or MAPK3, both in response to EGF and to cAMP. Its kinase activity is unsure. Some protein kinase activity has been detected in vitro, however the physiological relevance of this activity is unknown.
• Source: E. coli
• Species: Human
• Tag: C-His
• Protein length: 27-172aa
• Molecular Mass: 17.9 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: KSR1 kinase suppressor of ras 1 [ Homo sapiens (human) ]
• Official Symbol: KSR1
• Synonyms: KSR1; kinase suppressor of ras 1; kinase suppressor of ras, KSR; kinase suppressor of Ras 1; RSU2; KSR
• Gene ID: 8844
• mRNA Refseq: NM_014238
• Protein Refseq: NP_055053
• MIM: 601132
• UniProt ID: Q8IVT5