Recombinant Human Kinase Insert Domain Receptor (a type III receptor tyrosine kinase)

• Cat.No.: KDR-5447H
• Product Overview: Recombinant HumanKDR is produced as a non-chimeric protein in a monomeric form, which has aMW=116 kDa.

Specification

• Species: Human
• Source: Insect Cells
• Tag: Non
• Description: KDR, belonging to the family of receptortyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1),VEGFR-3 (Flt-4). Their expression is almost exclusively restricted toendothelial cells, but VEGFR-1 can also be found on monocytes. All KDR have seven immunoglobulin-like extracellulardomains, a single transmembrane region and an intracellular split tyrosinekinase domain. VEGFR-2 has a lower affinity for VEGF than the Flt-1 receptor,but a higher signaling activity. Mitogenic activity in endothelial cells ismainly mediated by VEGFR-2 leading to their proliferation. Differentialsplicing of the flt-1 gene leads to the formation of a secreted, solublevariant of VEGFR-1 (KDR). No naturally occurring, secreted formsof VEGFR-2 have so far been reported. The binding of VEGF165 to VEGFR-2 isdependent on heparin.
• Form: Lyophilized from abuffer of 20 mM Tris + 150 mM NaCl, pH 8.0.
• Molecular Weight: 116 kDa
• Purity: > 90% by SDS-PAGEand visualized by silver stain
• Endotoxin Level: < 0.1 ng/µg ofsKDR
• Biological Activity: The activity ofrecombinant human sVEGFR-2 was determined by its ability to abolish the bindingof iodinated VEGF to solid surfaces or cell surfaces. The ED50 for thiseffect is typically 10.0 ng/ml.
• Reconstitution: Centrifuge vialprior to opening. The lyophilized human sKDR is soluble in water and mostaqueous buffers. The lyophilized powder should be reconstituted in water orPBS to a concentration not lower than 100 µg/ml.
• Storage: The material isstable for greater than six months at -20°C to -80°C. After the first thawingit is recommended to aliquot the material in order to avoid repeated freeze-thawcycles. Store at 2-4°C not longer than 2 days.
• OfficialSymbol: KDR

Gene Information

• Gene Name: KDR kinase insert domainreceptor (a type III receptor tyrosine kinase) [ Homo sapiens ]
• Synonyms: KDR; kinase insertdomain receptor (a type III receptor tyrosine kinase); FLK1; CD309; VEGFR; VEGFR2;vascular endothelial growth factor receptor 2; soluble VEGFR2;OTTHUMP00000158928; fetal liver kinase 1; fetal liver kinase-1;protein-tyrosine kinase receptor Flk-1; tyrosine kinase growth factorreceptor; EC 2.7.10; EC 2.7.10.1
• Gene ID: 3791
• mRNA Refseq: NM_002253
• Protein Refseq: NP_002244
• MIM: 191306
• UniProt ID: P35968
• Chromosome Location: 4q11-q12
• Pathway: Angiogenesis;Cytokine-cytokine receptor interaction; Endocytosis; Focal Adhesion;HIF-2-alpha transcription factor network; Id Signaling Pathway; Integrins inangiogenesis; Neurophilin interactions with VEGF and VEGFR; Notch-mediatedHES/HEY network; S1P1 pathway; Signal Transduction; Signaling events mediatedby VEGFR1 and VEGFR2; VEGF binds to VEGFR leading to receptor dimerization;VEGF ligand-receptor interactions; VEGF signaling pathway
• Function: ATP binding; Hsp90protein binding; growth factor binding; integrin binding; nucleotide binding;protein binding; protein tyrosine kinase activity; receptor activity;receptor signaling protein tyrosine kinase activity; transmembrane receptorprotein tyrosine kinase activity; vascular endothelial growthfactor-activated receptor activity