Recombinant Human KEAP1 protein(Gln2-Cys624)

• Cat.No.: KEAP1-3232H
• Product Overview: Recombinant Human KEAP1 (Q14145) (Gln2-Cys624) was expressed and purified in Insect Cells with two additional amino acids (Gly & Pro ) at the N-terminus.

Specification

• Source: Insect Cells
• Species: Human
• Tag: Non
• Protein length: Gln2-Cys624
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 3mM DTT, 10% glycerol, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The secreted recombinant human KEAP1 consists of 625 amino acids and predicts a molecular mass of 69.7 KDa. The apparent molecular mass of the protein is approximately 64 KDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: KEAP1 kelch-like ECH-associated protein 1 [ Homo sapiens ]
• Official Symbol: KEAP1
• Synonyms: KEAP1; kelch-like ECH-associated protein 1; INrf2; KIAA0132; KLHL19; MGC1114; MGC4407; MGC9454; MGC10630; MGC20887; kelch-like protein 19; cytosolic inhibitor of Nrf2
• Gene ID: 9817
• mRNA Refseq: NM_012289
• Protein Refseq: NP_036421
• MIM: 606016
• UniProt ID: Q14145