Recombinant Human KDM2A protein, FLAG-tagged

• Cat.No.: KDM2A-46H
• Product Overview: Recombinant Human KDM2A(a.a: 2-700) fused with FLAG tag at N-terminal was expressed in Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: Flag
• Protein Length: 2-700 a.a.
• Description: Lysine (K)-specific demethylase 2A (KDM2A), also known as FBXL11, JHDM1A, CXXC8, nucleates at CpG islands and specifically demethylates "Lys-36" of histone H3, thereby playing a central role in histone code. Preferentially demethylates dimethylated H3 "Lys-36" residue while it has weak or no activity for mono- and tri-methylated H3 "Lys-36". May also recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation. Required to maintain the heterochromatic state. Associates with centromeres and represses transcription of small non-coding RNAs that are encoded by the clusters of satellite repeats at the centromere. Required to sustain centromeric integrity and genomic stability, particularly during mitosis.
• Form: In 25 mM Tris pH 8.0, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100 and 0.2 mg/ml 3× Flag peptide.
• Molecular Mass: 128 kDa
• Applications: Recombinant KDM2A / FBXL11 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Concentration: 0.25 μg/μl

Gene Information

• Gene Name: KDM2A lysine (K)-specific demethylase 2A [ Homo sapiens ]
• Official Symbol: KDM2A
• Synonyms: KDM2A; lysine (K)-specific demethylase 2A; F box and leucine rich repeat protein 11 , FBXL11; lysine-specific demethylase 2A; CXXC8; DKFZP434M1735; F box protein FBL11; FBL7; FBL11; FLJ00115; JHDM1A; jumonji C domain containing histone demethylase 1A; KIAA1004; LILINA; F-box/LRR-repeat protein 11; CXXC-type zinc finger protein 8; [Histone-H3]-lysine-36 demethylase 1A; F-box and leucine-rich repeat protein 11; jumonji C domain-containing histone demethylase 1A; jmjC domain-containing histone demethylation protein 1A; FBXL11; FLJ46431; DKFZp434M1735
• Gene ID: 22992
• mRNA Refseq: NM_012308
• Protein Refseq: NP_036440
• MIM: 605657
• UniProt ID: Q9Y2K7
• Chromosome Location: 11q13.1
• Function: DNA binding; histone demethylase activity (H3-K36 specific); metal ion binding; oxidoreductase activity; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; zinc ion binding