Recombinant Human KATNB1, T7-tagged

• Cat.No.: KATNB1-1543H
• Product Overview: Recombinant Human KATNB1 was expressed inE.Coliusing an N-terminal T7 tag. MW= 75.3 kDa.

Specification

• Cat. No.: KATNB1-1543H
• Description: Microtubules, polymers of α and β tubulin subunits, form the mitotic spindle of a dividing cell and help to organize membranous organelles during interphase. Katanin is a heterodimer that consists of a 60 kDa ATPase (p60 subunit A 1) and an 80 kDa accessory protein (p80 subunit B 1). The p60 subunit acts to sever and disassemble microtubules, while the p80 subunit targets the enzyme to the centrosome. Katanin is a member of the AAA family of ATPases.
• Source: E.Coli.
• Sequence: 1-655.
• Purity: ~95%.
• Specific Activity: n/aH.
• Preparation: 0.1mg/0.5 ml.
• Applications: E, WB, MS.
• Buffer: 10 mM Tris, pH 8.0, 0.1% Triton X-100, 0.002% NaN3, 10mM DTT.
• Storage: Store at -70˚C. As with any protein, exposing KATNB1 recombinant protein to repeated freeze/thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
• SDS-PAGE: SDS PAGE Analysis of KATNB1 Recombinant Protein. 4-20% SDS gradient gel. Coomassie blue staining.

Gene Information

• Gene Name: KATNB1 katanin p80 (WD repeat containing) subunit B 1 [ Homo sapiens ]
• Synonyms: KATNB1; katanin p80 (WD repeat containing) subunit B 1; KAT; katanin (80 kDa); katanin p80 subunit B 1; katanin p80 (WD40-containing) subunit B 1
• Gene ID: 10300
• mRNA Refseq: NM_005886
• Protein Refseq: NP_005877
• MIM: 602703
• UniProt ID: Q9BVA0
• Chromosome Location: 16q21
• Function: dynein binding; microtubule binding; microtubule-severing ATPase activity; protein heterodimerization activity