Recombinant Human Karyopherin Alpha 2 (RAG cohort 1, importin alpha 1), His-tagged

• Cat.No.: KPNA2-1389H
• Product Overview: Recombinant humanKPNA2 protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Karyopherinalpha2 (KPNA2) is a member of the karyopherin alpha family. This proteinplays a key role in the nuclear import of proteins with a classical nuclearlocalization signal (NLS). KPNA2 bound in vitro specifically and directly tosubstrates containing either a simple or bipartite NLS motif. It promoteddocking of import substrates to the nuclear envelope and together withrecombinant human Ran reconstituted complete nuclear protein import.
• Form: Liquid. 20mMTris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT.
• Molecular Weight: 60.5 kDa(553aa)
• Purity: > 90%by SDS - PAGE
• Concentration: 1 mg/ml(determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSHMSTNEN ANTPAARLHR FKNKGKDSTE MRRRRIEVNV ELRKAKKDDQMLKRRNVSSF PDDATSPLQE NRNNQGTVNW SVDDIVKGIN SSNVENQLQA TQAARKLLSR EKQPPIDNIIRAGLIPKFVS FLGRTDCSPI QFESAWALTN IASGTSEQTK VVVDGGAIPA FISLLASPHA HISEQAVWALGNIAGDGSVF RDLVIKYGAV DPLLALLAVP DMSSLACGYL RNLTWTLSNL CRNKNPAPPI DAVEQILPTLVRLLHHDDPE VLADTCWAIS YLTDGPNERI GMVVKTGVVP QLVKLLGASE LPIVTPALRA IGNIVTGTDEQTQVVIDAGA LAVFPSLLTN PKTNIQKEAT WTMSNITAGR QDQIQQVVNH GLVPFLVSVL SKADFKTQKEAVWAVTNYTS GGTVEQIVYL VHCGIIEPLM NLLTAKDTKI ILVILDAISN IFQAAENLGE TEKLSIMIEECGGLDKIEAL QNHENESVYK ASLSLIEKYF SVEEEEDQNV VPETTSEGYT FQVQDGAPGT FNF
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: KPNA2 karyopherin alpha 2 (RAGcohort 1, importin alpha 1) [ Homo sapiens ]
• Official Symbol: KPNA2
• Synonyms: KPNA2; karyopherin alpha 2 (RAG cohort 1,importin alpha 1); QIP2; RCH1; IPOA1; SRP1alpha; importin subunit alpha-2;pendulin; SRP1-alpha; RAG cohort 1; importin alpha 1; importin alpha 2;importin-alpha-P1; RAG cohort protein 1; karyopherin subunit alpha-2
• Gene ID: 3838
• mRNA Refseq: NM_002266
• Protein Refseq: NP_002257
• MIM: 600685
• UniProt ID: P52292
• Chromosome Location: 17q24.2
• Pathway: Influenza A; Regulation of cytoplasmic andnuclear SMAD2/3 signaling; Role of Calcineurin-dependent NFAT signaling inlymphocytes; Signaling events mediated by TCPTP; TNF-alpha/NF-kB SignalingPathway
• Function: histone deacetylase binding; nuclearlocalization sequence binding; protein binding; protein transporter activity