Recombinant Human ITGAM protein, His-tagged

• Cat.No.: ITGAM-28H
• Product Overview: Recombinant Human ITGAM(Phe17-Pro1104) fused with His tag at N-terminal was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• ProteinLength: 17-1104 a.a.
• Description: This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene.
• Form: Cell culture supernatant of transfected HEK293 cells, serum-free.
• Molecular Mass: Recombinant protein has a calculated molecular weight of about 123 kDa. The actual molecular weight may increase slightly due to the potential post-modifications (PTMs).
• Purity: Unpurified cell culture supernatant. HEK293 cells grown in serum-free medium were transfected with expression vector harboring target gene. The cell culture was harvested with centrifugation to remove cells. The cell culture supernatant containing mammalian cell protease inhibitor cocktail was aliquoted and stored at -80 centigrade immediately. The gene overexpression in culture supernatant was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the culture supernatant derived from HEK293 cells transfected with the empty expression vector was used as a negative control.
• Applications: Western blotting control, antibody validation (i.e., hybridoma screening, antibody pair screening), ELISA, EIA, dot blotting, immunoprecipitation (IP), protein array, protein functional assay, protein-protein interaction, post-translational modifications, etc.
• Storage: Upon arrival, the protein may be stored for 2 weeks at 4 centigrade. For long term storage, it is recommended to store at -20 centigrade or -80 centigrade in appropriate aliquots. Avoid repeated freeze-thaw cycles.
• Shipping: Ice packs. Including one vial of cell culture supernatant of HEK293 cells transfected with empty expression vector.

Gene Information

• Gene Name: ITGAM integrin, alpha M (complement component 3 receptor 3 subunit) [ Homo sapiens ]
• Official Symbol: ITGAM
• Synonyms: ITGAM; integrin, alpha M (complement component 3 receptor 3 subunit); CD11B, CR3A, integrin, alpha M (complement component receptor 3, alpha; also known as CD11b (p170), macrophage antigen alpha polypeptide); integrin alpha-M; CD11b; MAC 1; CR-3 alpha chain; antigen CD11b (p170); leukocyte adhesion receptor MO1; CD11 antigen-like family member B; macrophage antigen alpha polypeptide; cell surface glycoprotein MAC-1 subunit alpha; neutrophil adherence receptor alpha-M subunit; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6; MGC117044
• Gene ID: 3684
• mRNA Refseq: NM_000632
• Protein Refseq: NP_000623
• MIM: 120980
• UniProt ID: P11215