Recombinant Human Interleukin 1, Alpha, Fc Chimera

• Cat.No.: IL1A-109H
• Product Overview: Recombinant Human interleukin 1, alpha encoding the signal peptide and extracellular domain of human IL-1 Receptor I (IL-1RI) (aa 1-333) , was expressed in modifiedhuman 293 cells. It was fused to the Fc region of human IgG1 (aa 93-330).

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 1-333 a.a.
• Description: Interleukin 1, alpha Fc Chimera is an 80kDa type I transmembrane glycoprotein expressed on T cells and fibroblasts. Structurally the extracellular domain (ECD) of IL-1R1 contains 3 Immunoglobulin like domains. Glycosylation of IL-1R1has been shown to be necessary for optimal binding of IL-1, as blocking of glycosylation sites reduces binding to IL-1.
• Amino Acid Sequence: LEADKCK EREEKIIL VS SANEIDVR PCP LNPNEHK GTITWYK DDSKTP VSTEQA SRIHQ HKEKLWF VPAKVED SGHYY CVVR NSSY CLRIK ISAK FVE NEP NL CY NAQAIFKQK LPVAGDGGLVC PYMEFFK NENN EL PK LQ W YK DCKPLLLDNI HFSG VKDRLIVM NVAEKH RGNYTCH ASYTYLG KQYPITRVIE FIT LEE NKPT RPVIVSP ANETMEVDLG SQIQLICNVTGQL SDIAYWKWNGSVIDE DDPVLGE DYYSVE NPANKR RSTLI TVLNIS EIESRFYK HPFTCFAKN THGIDAAYIQ LIYPVTNRSS NTKV DKK VEPKS CDKTH TCPPCP AP ELLGGPSV FL FPP KPK DTLMISRTPEVTCVVVDVSHEDPE VKFNWY VDGVEV HNAKTK PREEQY NSTY RVVSVL TVLHQD WLNGK EYKCR VSNKALP APIEKTISKAKGQ PREPQ YTL PPSRDE LTKNQVSLTCL VKGFYPSDIAV EWESNGQPEN NYKTTPPVLDSD GSF FLYSKL TVDKS RWQQGNV FSCSVM HEALH NHYTQKSLSLSPGK.
• Molecular Mass: Interleukin 1, alpha Fc Chimera migrates as a broad band between 65 and 100 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with unmodified IL-1RI-Fc that has a predicted molecular mass of 63.6 kDa.
• pI: Interleukin 1, alpha Fc Chimera separates into a number of isoforms with a pI between 5 and 8 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified IL-1RI-Fc that has a predicted pI of 7.
• % Carbohydrate: Purified Interleukin 1, alpha Fc Chimera consists of 0-36% carbohydrate by weight.
• Glycosylation: Interleukin 1, alpha Fc Chimera has N-linked and may have O-linked oligosaccharides.
• Purity: >95%, as determined by SDS-PAGE and visualized by silver stain.
• Formulation: When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
• Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
• Storage: Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

Gene Information

• Gene Name: IL1A interleukin 1, alpha [ Homo sapiens ]
• Synonyms: IL1A; interleukin 1, alpha; IL1; IL-1A; IL1F1; IL1-ALPHA; preinterleukin 1 alpha; hematopoietin-1; pro-interleukin-1-alpha; Interleukin-1 alpha;IL-1 alpha; Hematopoietin-1; IL1A (IL1F1)
• Gene ID: 3552
• mRNA Refseq: NM_000573
• Protein Refseq: NP_000566
• MIM: 147760
• UniProt ID: P01583
• Chromosome Location: 2q14
• Pathway: Apoptosis; Cytokine-cytokine receptor interaction; Graft-versus-host disease; Hematopoietic cell lineage; MAPK signaling pathway; Prion diseases; Type I diabetes mellitus
• Function: cytokine activity; interleukin-1 receptor binding; protein binding