Recombinant Human Interferon Regulatory Factor 3

• Cat.No.: IRF3-27H
• Product Overview: Recombinant Human interferon regulatory factor 3 produced inE.Coliis a single, non-glycosylated, polypeptide chain containing 111 amino acids (1-112) and having a molecular mass of 13 kDa. The Interferon Regulatory Factor-3 is purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 1-112 a.a.
• Description: Members of the Interferon regulatory factor (IRF) family regulate gene expression critical to immune response, hemopoiesis, and proliferation. IRF-3 is a member of the IRF family, and is distinct from other family members. Its transcriptional activity is regulated solely by posttranslational modifications. It plays a crucial role in activation of innate immunity and inflammation in response to viral infection. IRF-3 mediates interferon-stimulated response element (isre) promoter activation. Functions as a molecular switch for antiviral activity. Dsrna generated during the course of an viral infection leads to IRF3 phosphorylation on the c-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with creb binding protein (crebbp) to form dsrna-activated factor 1 (draf1), a complex which activates the transcription of genes under the control of isre. The complex binds to the ie and prdiii regions on the ifn-alpha and ifn-beta promoters respectively. IRF-3 does not have any transcription activation domains.
• Amino Acid Sequence: MGTPKPRILP WLVSQLDLGQ LEGVAWVNKS RTRFRIPWKH GLRQDAQQED FGIFQAWAEA TGAYVPGRDK PDLPTWKRNF RSALNRKEGL RLAEDRSKDP HDPHKIYEFV NS.
• Physical Appearance: Sterile Filtered colorless solution.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: 1mg/ml in phosphate-buffered saline (PBS), pH 7.4.
• Storage: Liquid Interferon although stable at 10°Cfor 1 week, should be stored below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: IRF3 interferon regulatory factor 3 [ Homo sapiens ]
• Synonyms: IRF3; interferon regulatory factor 3; IRF-3
• Gene ID: 3661
• mRNA Refseq: NM_001571
• Protein Refseq: NP_001562
• MIM: 603734
• UniProt ID: Q14653
• Chromosome Location: 19q13.3-q13.4
• Pathway: RIG-I-like receptor signaling pathway; Toll-like receptor signaling pathway
• Function: RNA polymerase II transcription factor activity; transcription cofactor activity; transcription factor activity