Recombinant Human Interferon, Alpha 2, HQ-tagged

• Cat.No.: IFNA2-139H
• Product Overview: Recombinant human IFNA2molecular weight was 24 KDa.

Specification

• Species: Human
• Source: Barley Grain
• Tag: HQ
• Description: As a result ofglycosylation, the recombinant protein migrates with an apparent molecular massof 24 kDa in SDS-PAGE. ecombinant human IFNA2a contains 166 amino acids and a16 a.a. HQ tag for a total length of 182 a.a. and has a predicted molecular massof 21.5 kDa including HQ tag.
• Form: Sterile filteredthrough a 0.2 μm filter. Lyophilized from PBS, pH 7.2.
• Molecular Weight: 24 kDa
• Purity: > 95% by SDS-PAGEgel analysis. Purified product carries no pyrogenic or proinflammatory contaminants,as assayed with monocyte activation test using Human 10-plex Cytokine Assaymeasuring IL-6, TNF-alpha and IL-1beta induction.
• Endotoxin Level: Endotoxin level isless than 0.005 ng per μg of product (0.05 EU/μg) as measured by turbidimetrickinetic assay. Purified IFNA2a carries no pyrogenic or proinflammatory contaminants,as assayed with monocyte activation test using Human 10-plex Cytokine Assaymeasuring IL-6, TNF-alpha and IL-1beta induction.
• Biological Activity: Bioactivity isassayed by measuring the dose dependent cytotoxicity of human IFNA2a on TF-1in the presence of IL-4. The ED50 for this batch of recombinant human IFNA2awas determined to be 0.108 ng/ml, corresponding to specific activity of 9.26x 106 U/mg.
• Specific Activity: 9.26 x 106U/mg
• Reconstitution: Always centrifugethe vial before opening. It is recommended to reconstitute the lyophilizedprotein in sterile water to a concentration of no less than 100 μg/ml. Forlong term storage it is recommended to add a carrier protein (0.1% HSA orBSA). Please note that the addition of any carrier protein into this productmay produce unwanted endotoxin. This depends upon the particular applicationemployed.
• Storage: The lyophilizedprotein, though stable at room temperature for few weeks, is best stored at-20°C. Reconstituted protein should be used immediately or stored in workingaliquots at -20°C. Avoid repeated freeze-thaw cycles.
• OfficialSymbol: IFNA2

Gene Information

• Gene Name: IFNA2 interferon, alpha 2 [ Homosapiens ]
• Synonyms: IFNA2; interferon,alpha 2; IFNA;INFA2; MGC125764; MGC125765; IFN-alphaA; interferon alpha-2; leIF A; IFN-alpha-2;OTTHUMP00000021143; interferon alpha A; alpha-2a interferon; interferon alpha2b
• Gene ID: 3440
• mRNA Refseq: NM_000605
• Protein Refseq: NP_000596
• MIM: 147562
• UniProt ID: P01563
• Chromosome Location: 9p22
• Pathway: Autoimmune thyroiddisease; Cytokine Signaling in Immune system; Cytosolic DNA-sensing pathway;Factors involved in megakaryocyte development and platelet production;Hemostasis; Hepatitis C; Herpes simplex infection; Immune System; InfluenzaA; Innate Immune System; Interferon Signaling; Interferon alpha/betasignaling; Jak-STAT signaling pathway; Natural killer cell mediatedcytotoxicity; Regulation of autophagy
• Function: cytokine activity;interferon-alpha/beta receptor binding; protein binding