Recombinant Human Interferon, Alpha 2, His-tagged

• Cat.No.: IFNA2-009N
• Product Overview: This protein is produced in an expression system using Nicotiana sp. plants as an expression host

Specification

• Species: Human
• Source: Nicotiana Benthamiana
• Tag: His
• Description: At least 23 different variants of IFN-alpha are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end.
• Formulation: Lyophilized power, containing 80% SDS.
• Purity: Purity is assessed by SDS PAGE followed by Silver Stain Detection or Western blot analysis and by Capillary Electrophoresis using a 2100 Bioanalyzer.
• Quality analysis: Gel and capillary electrophoresis for estimation of purity. Mass spectrometry of tryptic digest products for identity determination.
• Amino acid sequence: The sequence of the first twelve N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Gln-Thr-His-Ser-Leu-Gly-Ser-Arg, conforming to the sequence of native human-IFα-2a. Other protein fragments detected by mass spectroscopy of tryptic digestion are highlighted in red on the following sequence representation.CDLPQTHSLGSRRTLMLLAQMRKISLFSCLKDRHDFGFPQEEFGNQFQKAETIPVLHEMIQQIFNLFSTKDSSAAWDETLLDKFYTELYQQLNDLEACVIQGVGVTETPLMNEDSILAVRKYFQRITLYLKEKKYSPCAWEVVRAEIMRSFSLSTNLQESLRSKE
• Endotoxin: < 0.0005 EU/µg by LAL test.
• Biological Activity: IFN-alpha 2a is fully biologically active when compared to standard. The specific activity was determined by a quantitative gene reported bioassay using human Type I interferon-sensitive cells. Compared with other recombinant IFα-2a, assayed in the same conditions, IFN-alpha 2a showed 6.1 × 108 Units/mg in reference to a viral resistance assay using bovine kidney MDBK cells.
• Storage: Product is presented as a lyophilized powder stable at 4°C. Adding 1 ml of distilled water to 1 mg of lyophilized powder gives a solution of 0.2 mg/ml of IFN Alpha-2a. After reconstitution in appropriate buffer aliquots should be stored at -70°C.

Gene Information

• Gene Name: IFNA2 interferon, alpha 2 [ Homo sapiens ]
• Official Symbol: INFA2
• Synonyms: IFNA; INFA2; MGC125764; MGC125765; Leukocyte interferon; B cell interferon, Type I interferon; IFNA2; IFN-α 2a; Interferon alpha-A; LeIF A; Interferon alpha-2; Interferon alpha-2 Precursor;interferon, alpha 2
• Gene ID: 3440
• mRNA Refseq: NM_000605
• Protein Refseq: NP_000596
• MIM: 147562
• UniProt ID: P01563
• Chromosome Location: 9p22
• Function: interferon-alpha/beta receptor binding