Recombinant Human Insulin-degrading Enzyme, His-tagged

• Cat.No.: IDE-255H
• Product Overview: Recombinant Human Insulysin produced inE.Coliis a single, non-glycosylated, polypeptide chain cotaning 990 amino acids (41-1018 a.a.) and having a molecular mass of 108.9 kDa. The Insulysin is fused to a 12 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 41-1018 a.a.
• Description: Insulysin is involved in cellular processing of insulin and in intercellular peptide signaling. Insulin-degrading enzyme is plays a role in the termination of the insulin response.
• Amino Acid Sequence: MHHHHHHAAG IPMNNPAIKR IGNHITKSPE DKREYRGLEL ANGIKVLLIS DPTTDKSSAA LDVHIGSLSD PPNIAGLSHF CEHMLFLGTK KYPKENEYSQ FLSEHAGSSN AFTSGEHTNY YFDVSHEHLE GALDRFAQFF LCPLFDESCK DREVNAVDSE HEKNVMNDAW RLFQLEKATG NPKHPFSKFG TGNKYTLETR PNQEGIDVRQ ELLKFHSAYY SSNLMAVCVL GRESLDDLTN LVVKLFSEVE NKNVPLPEFP EHPFQEEHLK QLYKIVPIKD IRNLYVTFPI PDLQKYYKSN PGHYLGHLIG HEGPGSLLSE LKSKGWVNTL VGGQKEGARG FMFFIINVDL TEEGLLHVED IILHMFQYIQ KLRAEGPQEW VFQECKDLNA VAFRFKDKER PRGYTSKIAG ILHYYPLEEV LTAEYLLEEF RPDLIEMVLD KLRPENVRVA IVSKSFEGKT DRTEEWYGTQ YKQEAIPDEV IKKWQNADLN GKFKLPTKNE FIPTNFEILP LEKEATPYPA LIKDTAMSKL WFKQDDKFFL PKACLNFEFF SPFAYVDPLH CNMAYLYLEL LKDSLNEYAY AAELAGLSYD LQNTIYGMYL SVKGYNDKQP ILLKKIIEKM ATFEIDEKRF EIIKEAYMRS LNNFRAEQPH QHAMYYLRLL MTEVAWTKDE LKEALDDVTL PRLKAFIPQL LSRLHIEALL HGNITKQAAL GIMQMVEDTL IEHAHTKPLL PSQLVRYREV QLPDRGWFVY QQRNEVHNNC GIEIYYQTDM QSTSENMFLE LFCQIISEPC FNTLRTKEQL GYIVFSGPRR ANGIQGLRFI IQSEKPPHYL ESRVEAFLIT MEKSIEDMTE EAFQKHIQAL AIRRLDKPKK LSAECAKYWG EIISQQYNFD RDNTEVAYLK TLTKEDIIKF YKEMLAVDAP RRHKVSVHVL AREMDSCPVV GEFPCQNDIN LSQAPALPQP EVIQNMTEFK RGLPLFPLVK PHINFMAAKL
• Physical Appearence: Sterile Filtered White lyophilized (freeze-dried) powder.
• Formulation: Lyophilized with no additives.
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Solubility: It is recommended to reconstitute the lyophilized Insulysin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Stability: Lyophilized Insulysin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Insulysin Recombinant should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: IDE insulin-degrading enzyme [ Homo sapiens ]
• Synonyms: IDE; insulin-degrading enzyme ; FLJ35968; INSULYSIN; insulinase; insulin protease; Abeta-degrading protease; EC 3.4.24.56; OTTHUMP00000020097; Insulysin
• Gene ID: 3416
• mRNA Refseq: NM_001165946
• Protein Refseq: NP_001159418
• MIM: 146680
• UniProt ID: P14735
• Chromosome Location: 10q23-q25
• Pathway: Alzheimer"s disease
• Function: ATP binding; ATPase activity; beta-endorphin binding; insulin binding; metal ion binding; metalloendopeptidase activity; nucleotide binding; peptidase activity; protein binding; protein homodimerization activity; signal transducer activity; zinc ion binding