Recombinant Human IL4 protein

• Cat.No.: IL4-504H
• Product Overview: Recombinant Human IL4 protein(P05112-1)(His25-Ser153) was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Non
• Protein length: His25-Ser153
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human IL-4, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 17.1 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: IL4 interleukin 4 [ Homo sapiens ]
• Official Symbol: IL4
• Synonyms: IL4; interleukin 4; interleukin-4; B cell growth factor 1; B_cell stimulatory factor 1; BCGF 1; BCGF1; BSF1; IL 4; lymphocyte stimulatory factor 1; MGC79402; binetrakin; pitrakinra; IL-4; BSF-1; BCGF-1
• Gene ID: 3565
• mRNA Refseq: NM_000589
• Protein Refseq: NP_000580
• MIM: 147780
• UniProt ID: P05112

Reviews

10/17/2021

By testing immunogenicity in animal models, detect whether an immune response is induced.

11/09/2020

The structure of the recombinant protein was analyzed by nuclear magnetic resonance (NMR), X-ray crystallography or electron microscopy, and the results showed that the protein was stable.

05/06/2020

This protein has good stability, and the test results of heat resistance, acid and alkali resistance and protease degradation resistance are good.