Recombinant Human IL12B & IL23A protein(Arg20-Pro189&Ile23-Ser328), His-Avi-tagged, Biotinylated
Cat.No. : | IL12B & IL23A-1261H |
Product Overview : | Biotinylated Recombinant Human IL12B & IL23A protein(Arg20-Pro189&Ile23-Ser328)(Q9NPF7&P29460) is expressed from HEK293 with His tag and Avi tag at the C-Terminus. |
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Species : | Human |
Source : | HEK293 |
Tag : | Avi&His |
Protein Length : | Arg20-Pro189&Ile23-Ser328 |
Form : | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized Biotinylated Human IL-23 alpha&IL-12 beta, His Tag at 0.5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-IL-23 Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA. |
Molecular Mass : | The protein has a predicted MW of 21.7 (IL-23 alpha)&34.7 (IL-12 beta) kDa. Due to glycosylation,the protein migrates to 24 kDa (IL-23 alpha) and 40-50 kDa (IL-12 beta) kDa based on Tris-Bis PAGE result. |
Endotoxin : | Less than 1EU per μg by the LAL method. |
Purity : | > 95% as determined by Tris-Bis PAGE > 95% as determined by HPLC |
Storage : | -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Reconstitution : | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Gene Name | IL12B interleukin 12B [ Homo sapiens (human) ] |
Official Symbol | IL12B & IL23A |
Synonyms | CLMF; NKSF; CLMF2; IMD28; IMD29; NKSF2; IL-12B; IL23A |
Gene ID | 3593 |
mRNA Refseq | NM_002187 |
Protein Refseq | NP_002178 |
MIM | 161561 |
UniProt ID | P29460 |
◆ Recombinant Proteins | ||
IL12B & IL23A-1156R | Recombinant Rabbit IL12B & IL23A protein(Met1-Asn324 & Met1-Pro191), His-tagged | +Inquiry |
IL12B & IL23A-1261H | Recombinant Human IL12B & IL23A protein(Arg20-Pro189&Ile23-Ser328), His-Avi-tagged, Biotinylated | +Inquiry |
Not For Human Consumption!
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