Recombinant Human IL12A

• Cat.No.: IL12A-112H
• Product Overview: Recombinant Human interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) sequence (containing the signal peptide and the mature IL-12 alpha chain sequence, and the signal peptide and the mature IL-12 beta chain sequence) was expressed in modifiedhuman 293 cells.

Specification

• Cat. No.: IL12A-112H
• Description: Human interleukin 12 (IL-12, IL12) is a pro-inflammatory cytokine that consists of a heterodimer comprising two disulfide-linked subunits, the alpha chain (the p35 subunit, IL-12A) and the beta chain (the p40 subunit, IL-12B).
• Source: Human 293 cells.
• Amino Acid Sequence: HGTELPSPPSVWFEA EFFHHILHWTPIPNQSES TCYEVALLRYGIESW NSISNCSQ TLSYDLT AVTL DLYHSNG YRARVRAV DGSRHSNWTV TNTRFSVDEVT LT V G SVNLEIH NGFIL GKIQLPR PK MAPANDTY ESIFSHF REYEIAIR KVP GN F T F THKKVK HENFSLLTSGEVGE FCV QVK PSV ASRS NKGMWSK EE CIS LTRQY FTGIPKVDKKV EPK SC DKT HTC PPCPA PELLGGPSV FLFP PKP KDT LM IS RTP EVT CVVVDVS HED PEVKF NWYVDGV EVHNAKTKPREEQYNST YRV VSV L TVLHQ DWLNG EYKCRVS NKALPAPI EKTISKAK GQPREPQ VYTLPPSR DELTK NQVSL TCLVK GFYPSD IAVEWE SNGQPE NNYKTTP PVLDSDGSFFLY SKL TV DKSRWQ QGNVFSCSVMH EALHNHYT QKSLSLSPGK.
• Molecular Mass: IL-12A is a disulfide-linked heterodimeric glycoprotein, consisting of an alpha and beta chain.IL-12A migrates as multiple bands between 30 and 45 kDa in SDS-PAGE due to post-translational modifications, in particular glycosylation. The IL-12A alpha chain migrates as bands between 30 and 35 kDa. This compares with the unmodified IL-12 alpha chain that has a predicted molecular mass of 22.5 kDa. The IL-12A beta chain migrates as bands between 35 and 45 kDa. This compares with the unmodified IL-12 beta chain that has a predicted molecular mass of 34.7 kDa.
• PI: IL-12 separates into a number of isoforms with a pI between 3.5 and 9.5 in 2D PAGE due to post-translational modifications, in particular glycosylation. IL-12 alpha chain separates into multiple isoforms with a pI between 3.5 and 9.5. This compares with the unmodified IL-12 alpha chain that has a predicted pI of 6.1. The Symansis IL-12 beta chain separates into multiple isoforms with a pI between 5.0 and 9.0. This compares with the unmodified IL-12 beta chain that has a predicted pI of 5.4.
• % Carbohydrate: Purified IL-12 alpha chain consists of 15-35% carbohydrate by weight. purified IL-12 beta chain consists of 0-25% carbohydrate by weight.
• Glycosylation: IL-12 has N- and O-glycosylation.
• Purity: >95%, as determined by SDS-PAGE and visualized by silver stain.
• Formulation: When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
• Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
• Storage: Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
• Tag: Non

Gene Information

• Gene Name: interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
• Synonyms: IL12A; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); P35; CLMF; NFSK; NKSF1; IL-12A; natural killer cell stimulatory factor 1, 35 kD subunit; cytotoxic lymphocyte maturation factor 1, p35; interleukin 12, p35; IL-12, subunit p35; NF cell stimulatory factor chain 1; interleukin-12 alpha chain;Cytotoxic lymphocyte maturation factor 35 kDa subunit
• Gene ID: 3592
• mRNA Refseq: NM_000822
• Protein Refseq: NP_000873
• MIM: 161560
• UniProt ID: P29459
• Chromosome Location: 3q25.33-q26
• Pathway: Allograft rejection; Asthma; Autoimmune thyroid disease; Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Systemic lupus erythematosus; T cell receptor signaling pathway
• Function: cytokine activity; growth factor activity; interleukin-12 receptor binding ; interleukin-12 beta subunit binding ; interleukin-27 binding protein binding; protein heterodimerization activity