Recombinant Human IGF1R Protein, His/GST-tagged, Alexa Fluor 555 conjugated

• Cat.No.: IGF1R-50HAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant human IGF1R (NP_000866.1) cytoplasmic domain (Met 954-Cys 1367), fused with the N-terminal polyhistidine-tagged GST tag, was produced in Baculovirus-Insect cells.

Specification

• Source: Insect Cells
• Species: Human
• Tag: His&GST
• Form: Lyophilized
• Molecular Mass: The recombinant human IGF1R (aa 954-1367) /GST chimera consists of 651 amino acids and has a calculated molecular mass of 74.6 kDa. It migrates as an approximately 70 kDa band in SDS-PAGE under reducing conditions.
• Protein length: 651
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 20% glycerol, 0.3 mM DTT
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: IGF1R insulin-like growth factor 1 receptor [ Homo sapiens ]
• Official Symbol: IGF1R
• Gene ID: 3480
• mRNA Refseq: NM_000875
• Protein Refseq: NP_000866
• MIM: 147370
• UniProt ID: P08069