Recombinant Human IDS Protein, Full Length, C-10×His tagged

• Cat.No.: IDS-18HFL
• Product Overview: Recombinant Human IDS Protein (Full Length) with C-10×His tag was expressed in Mouse myeloma cell line.

Specification

• Species: Human
• Source: Mouse myeloma cell
• Tag: His
• Protein Length: Full Length
• Description: This gene encodes a member of the sulfatase family of proteins. The encoded preproprotein is proteolytically processed to generate two polypeptide chains. This enzyme is involved in the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this gene are associated with the X-linked lysosomal storage disease mucopolysaccharidosis type II, also known as Hunter syndrome. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
• Bio-activity: Measured by its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS). The specific activity is > 1.0 pmol/min/μg, as measured under the described conditions.
• Molecular Mass: Predicted Molecular Mass: 61 kDa
  SDS-PAGE: Multiple bands between 74-91 kDa, reducing conditions
• Endotoxin: <1.0 EU/μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• N-terminal Sequence Analysis: Ser26
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  3 months, -20 to -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Shipping: The product is shipped with polar packs.
• References: 1. Wilson, P.J. et al. (1990) Proc. Natl. Acad. Sci. USA 87:8531.
  2. Parenti, G. et al. (1997) Curr. Opin. Genet. & Dev. 7:386.
  3. Neufeld, E.F. and Muenzer, J. (2001) in The Metabolic and Molecular Basis of Inherited Disease, Scriver, C.R. et al. (eds.) pp. 3421, New York, McGraw-Hill.

Gene Information

• Gene Name: IDS iduronate 2-sulfatase [ Homo sapiens (human) ]
• Official Symbol: IDS
• Synonyms: IDS; iduronate 2-sulfatase; ID2S; MPS2; SIDS; iduronate 2-sulfatase; alpha-L-iduronate sulfate sulfatase; iduronate 2-sulfatase 14 kDa chain; iduronate 2-sulfatase 42 kDa chain; idursulfase; EC 3.1.6.13
• Gene ID: 3423
• mRNA Refseq: NM_000202
• Protein Refseq: NP_000193
• MIM: 300823
• UniProt ID: P22304