Recombinant Human ICOS protein, hFc-tagged

Cat.No. : ICOS-3244H
Product Overview : Recombinant Human ICOS protein(Q9Y6W8-1)(Glu21-Phe141), fused with C-terminal hFc tag, was expressed in HEK293.
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Source : HEK293
Species : Human
Tag : C-hFc
Protein length : Glu21-Phe141
Form : Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized Human B7-H2, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA.
Molecular Mass : The protein has a predicted MW of 40.5 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.
Endotoxin : Less than 1EU per μg by the LAL method.
Purity : > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Storage : Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Reconstitution : Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Gene Name ICOS inducible T-cell co-stimulator [ Homo sapiens ]
Official Symbol ICOS
Synonyms ICOS; inducible T-cell co-stimulator; inducible T-cell costimulator; activation inducible lymphocyte immunomediatory molecule; AILIM; CD278; inducible costimulator; activation-inducible lymphocyte immunomediatory molecule; CVID1; MGC39850;
Gene ID 29851
mRNA Refseq NM_012092
Protein Refseq NP_036224
MIM 604558
UniProt ID Q9Y6W8

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